Totally 35 patients listed for cataract surgery (controls: n=12, age: 67.4±13.6y) or trabeculectomy for POAG (n=23, age: 72.5±8.3y) were included. AH samples of those patients were obtained during cataract surgery or trabeculectomy. AH samples were subsequently pooled into the experimental groups under equal contribution in terms of protein amount of each individual patient. Protein samples were analyzed by a linear trap quadrupol Orbitrap Mass Spectrometry device with an upstream liquid chromatography system. The obtained raw data were analyzed using the Maxquant proteome software and compared.
Proteins with a fold-change ratio higher than a cut-off of 2 were considered as noticeably altered.A total number of 175 proteins could be identified out of the AH from POAG and cataract by means of quantitative mass spectrometric analysis.
Apolipoprotein D (fold change, 3.16 times), complement C3 (2.96), pigment epithelium-derived factor (2.86), dickkopf-related protein 3 (2.18) and wingless-related integration (Wnt) inhibitory factor 1 (2.35) were significantly upregulated within the AH of glaucoma compared to cataract serving as controls.AH provides a tool to analyze changes in glaucoma and shows striking changes in Wnt signaling inhibitory molecules and other proteins.
KT 5720 |
B9002-.5 |
ApexBio |
500 µg |
EUR 818 |
KT 5823 |
B6673-.05 |
ApexBio |
50 µg |
EUR 123 |
KT 5823 |
B6673-.1 |
ApexBio |
100 µg |
EUR 190 |
KT 5823 |
B6673-.25 |
ApexBio |
250 µg |
EUR 361 |
Plasminogen Receptor (KT) (PLGRKT) Antibody |
20-abx318476 |
Abbexa |
-
EUR 411.00
-
EUR 1845.00
-
EUR 599.00
-
EUR 182.00
-
EUR 300.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
KT 5823 (PKG kinase inhibitor) |
SIH-458-100UG |
Stressmarq |
100 µg |
EUR 205 |
|
Description: The substance KT 5823 is a pkg kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white solid which is soluble in DMSO (20 mg/ml), dimethyl formamide (20 mg/ml) and ethyl acetate (5 mg/ml). |
KT 5823 (PKG kinase inhibitor) |
SIH-458-500UG |
Stressmarq |
500 µg |
EUR 569 |
|
Description: The substance KT 5823 is a pkg kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is white solid which is soluble in DMSO (20 mg/ml), dimethyl formamide (20 mg/ml) and ethyl acetate (5 mg/ml). |
DHRS2 antibody |
70R-16822 |
Fitzgerald |
50 ul |
EUR 435 |
Description: Rabbit polyclonal DHRS2 antibody |
Rat Chondroitin Sulfate Epitope 3B3(CS3B3 ELISA Kt |
GA-E0451RT-48T |
GenAsia Biotech |
48T |
EUR 317 |
Rat Chondroitin Sulfate Epitope 3B3(CS3B3 ELISA Kt |
GA-E0451RT-96T |
GenAsia Biotech |
96T |
EUR 496 |
Factor XI Antibody Pair |
55R-FAC11-KT |
Fitzgerald |
5 plates |
EUR 745 |
Description: Factor XI antigen Matched Pair antibody Set for ELISA |
Factor IX Antibody Pair |
55R-FAC9-KT |
Fitzgerald |
5 plates |
EUR 760 |
Description: Factor IX antigen Matched Pair antibody Set for ELISA |
Fibrinogen Matched Pair antibody Set |
55R-FIBRINO-KT |
Fitzgerald |
set |
EUR 833 |
Description: Fibrinogen Matched Pair antibody Set for detection of Human Fibrinogen in the research laboratory |
Mouse RANKL ELISA Kit |
55R-MRANKL-KT |
Fitzgerald |
1 kit |
EUR 658 |
Description: ELISA kit for detection of Mouse RANKL in the research laboratory |
VWF Antibody Pair |
55R-VWF-KT |
Fitzgerald |
5 plates |
EUR 775 |
Description: VWF antigen Matched Pair antibody Set for ELISA |